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Dr. Peter Quigley, LCC Chancellor A little learning, a little supervision....
Participants of the 2005 workshop sponsored by USDA-CSREES Business Incubation Project. Front row from left, Christine Messah-Oh (Leeward CC), Victor Wong (Bioxene), Roy Matsumoto (Eurus Genomics), Mayumi Kudo (Eurus), Miwako Osawa (Eurus), Ike Miguel (LCC ), Kabi Neupane (LCC). Back row from left, Yuka Hiroi (Eurus), Shizuka Shinohara (Eurus), Traci Harada (Hawaii Biotech), Anna Maeno (Eurus), Go Maeno (Eurus) Chancellor Quigley welcomes the workshop particiants Each day's activities included lectures and hands-on laboratory exercises. Learning to culture bacteria in a biosafety cabinet Getting ready to grind plant tissue in liquid nitrogen for DNA extraction Safety first...getting ready to visualize DNA on a UV transilluminator Some cultural and language training on the side!! Quantification of DNA using a Dual Beam Spectrophotometer University of Hawaii's Biosafety Officer Hubert Olipares discusses biosafety Using molecular models for learning Victor Wong, CEO of Bioxene Inc., is pipetting DNA sample.
Participants of the 2005 workshop sponsored by USDA-CSREES Business Incubation Project. Front row from left, Christine Messah-Oh (Leeward CC), Victor Wong (Bioxene), Roy Matsumoto (Eurus Genomics), Mayumi Kudo (Eurus), Miwako Osawa (Eurus), Ike Miguel (LCC ), Kabi Neupane (LCC). Back row from left, Yuka Hiroi (Eurus), Shizuka Shinohara (Eurus), Traci Harada (Hawaii Biotech), Anna Maeno (Eurus), Go Maeno (Eurus)
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Hands-on Workshop on
Biotechnology for Beginners
This week-long workshop included lectures and
wet-labs specifically designed to give beginners a hands-on experience
in a biotechnology laboratory. Participants extracted genomic and
plasmid DNA, performed restriction digestion, Agarose gel
electrophoresis, bacterial transformation, gene cloning, and learnt the
theory and practice of PCR technology. The workshop was organized by Dr.
Kabi R. Neupane at Leeward Community College. Lectures and laboratories
are adapted from ABE Summer Workshop. Financial support for this
workshop was provided by the
University of Hawai‘i
Statewide Agriculture Education and Agribusiness Incubation Consortium
Project.
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Workshop
Schedule |
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Monday August
8, 2005 |
8:30 AM -9:00
AM |
Registration,
Coffee, Introductions |
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9:00 AM -9:15
AM |
Welcome
remarks- Dr. Peter Quigley, Acting Chancellor, LCC |
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9:00 AM -10:00
AM |
Overview of
workshop and introduction to the laboratory- Kabi Neupane |
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10:00 AM -12:00
Noon |
Lab Activities:
aseptic techniques, media and antibiotics |
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10:30 AM -12:00
Noon |
Activity: DNA
structure |
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12:00 Noon
-1:30 PM |
Lunch Break |
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1:30 PM- 2:30
PM |
Recombinant DNA
technology - Kabi Neupane |
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2:30 PM- 4:00
PM |
Lab Activities:
Agarose gel electrophoresis |
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2:30 PM-4:30 PM |
Bacterial
transformation |
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4:30 PM-5:00 PM |
Group
Discussion/Summary |
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Tuesday August
9, 2005 |
8:00 AM -8:30
AM |
Coffee |
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8:30 AM -9:30
AM |
Introduction to
restriction enzymes- Kabi Neupane |
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9:30 AM -10:30
AM |
Lab Activity:
Restriction digestion |
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10:30 AM -11:30
Noon |
Lab Activity:
Preparation of 0.8% agarose gel |
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11:30 AM -12:00
Noon |
Gel
electrophoresis |
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12:00 Noon
-1:30 PM |
Lunch Break |
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1:30 PM- 4:00
PM |
Isolation and
quantitation of genomic DNA from plants- Kabi Neupane |
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4:00 PM-4:30 PM |
Growing
bacterial clones for plasmid isolation |
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4:30 PM-5:00 PM |
Group
Discussion/Summary |
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Wednesday
August 10, 2005 |
8:00 AM -8:30
AM |
Coffee |
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8:30 AM -9:00
AM |
Introduction to
plasmid vectors and plasmid DNA isolation- Kabi Neupane |
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9:00 AM -12:00
AM |
Lab Activity:
Plasmid DNA isolation, restriction digestion and gel
electrophoresis |
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12:00 Noon
-1:30 PM |
Lunch Break |
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1:30 PM- 2:30
PM |
Introduction to
Polymerase chain reaction- Kabi Neupane |
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2:30 PM-4:30 PM |
Lab Activity:
Set up and run polymerase chain reaction (PCR) |
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4:30 PM-5:00 PM |
Group
Discussion/Summary |
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Thursday August
11, 2005 |
8:00 AM -8:30
AM |
Coffee |
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8:30 AM -9:30
AM |
TDNA
Mutagenesis and mapping- Kabi Neupane |
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9:00 AM -12:00
AM |
Lab
Activity: TDNA mutagenesis and mapping |
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12:00 Noon
-1:30 PM |
Lunch Break |
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1:30 PM-4:30 PM |
Primer Design
and bioinformatics lab |
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4:30 PM-5:00 PM |
Group
Discussion/Summary |
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Friday
August 12, 2005 |
8:00 AM -8:30
AM |
Coffee |
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8:30 AM -10:00
AM |
Lab Activity:
Gel Electrophoresis of TDNA mapping products |
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10:00 AM -11:00
AM |
DNA sequencing
and Single nucleotide polymorphisms- Kabi Neupane |
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11:00 AM -12:00
Noon |
Group
discussion (lab safety, PowerPoint presentation) |
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12:00 Noon
-1:30 PM |
Lunch Break |
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1:30 PM-3:30 PM |
Prepare group
presentations |
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3:30 PM-4:30 PM |
Presentation of
results |
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4:30 PM-5:00 PM |
Group
Discussion and evaluation |
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